Osteoporosis and Osteoarthritis 2nd Edition by Jennifer Westendorf, Andre van Wijnen 149391619X 9781493916191

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Product details:

ISBN 10: 149391619X 

ISBN 13: 9781493916191

Author: Jennifer J. Westendorf, Andre J. van Wijnen

In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,  Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.

Table of contents:

Isolation Of Osteocytes From Mature and Aged Murine Bone

Primary Murine Growth Plate and Articular Chondrocyte Isolation and Culture

Isolating Endosteal Mesenchymal Progenitors From Rodent Long Bones

Engineering Cartilage Tissue By Pellet Co-Culture Of Chondrocytes And Mesenchymal Stem Cells

Generation Of Induced Pluripotent Stem Cells

Profiling Histone Modifications By Chromatin Immunoprecipitation Coupled To Deep Sequencing In Skeletal Cells

Identification Of Micrornas In Human Plasma

Immunohistochemistry Of Skeletal Tissues

In Vivo Axial Loading Of The Mouse Tibia

Four Point Bending Protocols To Study The Effects Of Dynamic Strain In Osteoblastic Cells In Vitro

Epic-µCT Imaging Of Articular Cartilage

Mouse Models Of Osteoarthritis: Surgical Model Of Post-Traumatic Osteoarthritis Induced By Destabilization Of The Medial Meniscus

Assessment Of Knee Joint Pain In Experimental Rodent Models Of Osteoarthritis

Induction Of Fully Stabilized Cortical Bone Defects To Study Intramembranous Bone Regeneration

Surgical Procedures And Experimental Outcomes Of Closed Fractures In Rodent Models

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Tags: Jennifer Westendorf, Andre van Wijnen, Osteoporosis, Osteoarthritis