Photodynamic Therapy Methods and Protocols 1st Edition by Thomas J Dougherty – Ebook PDF Instant Download/Delivery:1607616971, 9781607616979
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Product details:
ISBN 10: 1607616971
ISBN 13: 9781607616979
Author: Thomas J. Dougherty
Photodynamic Therapy: Methods and Protocols is a comprehensive guide that delves into the methodologies and protocols pertinent to photodynamic therapy (PDT) research. Edited by Charles J. Gomer, this volume is part of the Methods in Molecular Biology series and was first published in 2010. The book compiles contributions from leading PDT scientists and clinicians, offering a detailed collection of methods and protocols related to mechanistic studies, dosimetry, preclinical models, and clinical procedures in PDT research. Reflecting the expanding scope of PDT, the content moves beyond traditional concepts to explore various signal transduction and cell death pathways involved in treatment outcomes. Each chapter provides an introduction to its topic, a list of necessary materials and reagents, step-by-step laboratory protocols, and notes highlighting troubleshooting tips and potential pitfalls.
Table of contents:
Introduction
Thomas J. Dougherty
An overview of the development and principles of photodynamic therapy.
Death and Survival Signals in Photodynamic Therapy
Michael Dewaele, Tom Verfaillie, Wim Martinet, Patrizia Agostinis
Exploration of cellular pathways influencing cell fate post-PDT.
Photodynamic Therapy and Cell Death Pathways
David Kessel, Nancy L. Oleinick
Analysis of mechanisms leading to apoptosis and other forms of cell death induced by PDT.
Identification of MAP Kinase Pathways Involved in COX-2 Expression Following Photofrin Photodynamic Therapy
Marian Luna, Angela Ferrario, Sam Wong, Charles J. Gomer
Investigation into the molecular pathways activated during PDT and their implications.
Metronomic PDT and Cell Death Pathways
Gurmit Singh, Omar Alqawi, Myrna Espiritu
Study of low-dose, continuous PDT and its effects on tumor cells.
How to Monitor NF-κB Activation After Photodynamic Therapy
Isabelle Coupienne, Jacques Piette, Sébastien Bontems
Methods to assess the activation of NF-κB, a key transcription factor, following PDT.
Application of 5-Aminolevulinic Acid and Its Derivatives for Photodynamic Therapy In Vitro and In Vivo
Asta Juzeniene, Petras Juzenas, Johan Moan
Protocols for using 5-ALA and its derivatives in experimental and clinical settings.
Hypoxia and Perfusion Labeling During Photodynamic Therapy
Theresa M. Busch
Techniques to evaluate oxygen levels and blood flow in tissues undergoing PDT.
Targeting the Tumor Microenvironment Using Photodynamic Therapy Combined with Inhibitors of Cyclooxygenase-2 or Vascular Endothelial Growth Factor
Angela Ferrario, Charles J. Gomer
Strategies to enhance PDT efficacy by modulating the tumor microenvironment.
Photochemical Internalization (PCI): A Technology for Drug Delivery
Kristian Berg, Anette Weyergang, Lina Prasmickaite, Anette Bonsted, Anders Høgset, Marie-Therese R. Strand, Ernst Wagner, Pål K. Selbo
Description of PCI techniques to improve intracellular delivery of therapeutic agents.
Photodynamic Therapy-Generated Cancer Vaccines
Mladen Korbelik
Exploration of PDT’s potential to stimulate anti-tumor immune responses.
Antimicrobial Photodynamic Inactivation and Photodynamic Therapy for Infections
Liyi Huang, Tianhong Dai, Michael R. Hamblin
Application of PDT in treating microbial infections.
Photodynamic Therapy of Bacterial and Fungal Biofilm Infections
Merrill A. Biel
Approaches to disrupt and eradicate biofilms using PDT.
Calculation of Cellular Oxygen Concentration for Photodynamic Therapy In Vitro
Michael S. Patterson, Emma Mazurek
Methods to determine oxygen availability in cell cultures during PDT.
Fluorescent Molecular Imaging and Dosimetry Tools in Photodynamic Therapy
Brian W. Pogue, Kimberley S. Samkoe, Summer L. Gibbs-Strauss, Scott C. Davis
Techniques for imaging and measuring PDT dose delivery.
Bifunctional Agents for Imaging and Therapy
Ravindra K. Pandey, Nadine S. James, Yihui Chen, Joseph Missert, Munawar Sajjad
Development of agents that combine diagnostic and therapeutic functions.
Photodynamic Diagnosis and Therapy and the Brain
Herwig Kostron
Application of PDT in neurosurgery and brain tumor treatment.
Photodynamic Therapy of Head and Neck Cancers
Merrill A. Biel
Clinical protocols and outcomes for treating head and neck malignancies with PDT
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