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Plant Receptor Kinases Methods and Protocols 1st Edition by Reidunn Birgitta Aalen 1493983822 9781493983827

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Plant Receptor Kinases 1st Edition Reidunn Birgitta Aalen Digital Instant Download

Author(s): Reidunn Birgitta Aalen
ISBN(s): 9781493983827, 1493983822
Edition: 1st
File Details: PDF, 5.25 MB
Year: 2018
Language: english
SKU: EB-47740298 Category: Tag:

Plant Receptor Kinases Methods and Protocols 1st Edition by Reidunn Birgitta Aalen – Ebook PDF Instant Download/Delivery: 1493983822, 9781493983827

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Product details:

ISBN 10: 1493983822 

ISBN 13: 9781493983827

Author: Reidunn Birgitta Aalen

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents:

  1. Expression of Plant Receptor Kinases in E. coli

  2. Expression of Plant Receptor Kinases in Yeast

  3. Expression of Plant Receptor Kinases in Tobacco BY‑2 Cells

  4. Cell‑free Synthesis of Plant Receptor Kinases

  5. Purification of Plant Receptor Kinases from Plant Plasma Membranes

  6. Photoaffinity Labeling of Plant Receptor Kinases

  7. Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases

  8. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis

  9. In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping

  10. Co‑immunoprecipitation of Plant Receptor Kinases

  11. Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell‑free Systems

  12. Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners

  13. In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases

  14. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases

  15. Pull‑Down Assay to Characterize Ca²⁺/Calmodulin Binding to Plant Receptor Kinases

  16. Use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases

  17. Detection and Analyses of Endocytosis of Plant Receptor Kinases

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Tags: Reidunn Birgitta Aalen, Receptor, Plant, Methods