Plant Virology Protocols From Viral Sequence to Protein Function 2nd Edition by Gary Foster, Elisabeth Johansen, Yiguo Hong, Peter Nagy 1597451029 9781597451024

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ISBN 10: 1597451029

ISBN 13: 9781597451024

Author: Gary D. Foster, I. Elisabeth Johansen, Yiguo Hong, Peter D. Nagy

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.

Plant Virology Protocols From Viral Sequence to Protein Function 2nd Table of contents:

Plant-virus interactions / Peter Palukaitis, John P. Carr, and James E. Schoelz

Role of capsid proteins / John F. Bol

Role of plant virus movement proteins / Michael Taliansky, Lesley Torrance, and Natalia O. Kalinina

Multiple roles of viral replication proteins in plant RNA virus replication / Peter D. Nagy and Judit Pogany

Role of silencing suppressor proteins / József Burgyán

Role of vector-transmission proteins / Véronique Ziegler-Graff and Véronique Brault

In vivo analyses of viral RNA translation / William R. Staplin and W. Allen Miller

In vitro analysis of translation enhancers / Aurélie M. Rakotondrafara and W. Allen Miller

Identification of plant virus IRES / Sek-Man Wong, Dora Chin-Yen Koh, and Dingxiang Liu

Analysis of geminivirus DNA replication by 2-D gel / Keith Saunders

Begomoviruses : molecular cloning and identification of replication origin / Lilian H. Florentino [and others]

Analysis of viroid replication / Ricardo Flores [and others]

Biochemical analyses of the interactions between viral polymerases and RNAs / Young-Chan Kim and C. Cheng Kao

In situ detection of plant viruses and virus-specific products / Andrew J. Maule and Zoltán Havelda

Detection of siRNAs and miRNAs / Sakari Kauppinen and Zoltán Havelda

Cloning of short interfering RNAs from virus-infected plants / Thien X. Ho [and others]. Solution structure probing of RNA structures / Marc R. Fabian and K. Andrew White

RNA encapsidation assay / Padmanaban Annamalai and A.L.N. Rao

Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus / K.S. Rajendran and Peter D. Nagy

Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis / Raúl C. Gomila and Lee Gehrke

Probing interactions between plant virus movement proteins and nucleic acids / Tzvi Tzfira and Vitaly Citovsky

Movement profiles : a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins / Kateryna Trutnyeva, Pia Ruggenthaler, and Elisabeth Waigmann

Analysis of siRNA-suppressor of gene silencing interactions / Lórant Lakatos and József Burgyán

Phosphorylation analysis of plant viral proteins / Kristiina M. Mäkinen and Konstantin I. Ivanov

Analysis of interactions between viral replicase proteins and plant intracellular membranes / Hélène Sanfaçon and Guangzhi Zhang

Membrane and protein dynamics in virus-infected plant cells / Michael Goodin, Romit Chakrabarty, and Sharon Yelton

Site-directed mutagenesis of whole viral genomes / Li Liu and George P. Lomonossoff

Viral protein-nucleic acid interaction : south (north)-western blot / Huanting Liu

Protein-protein interactions : the yeast two-hybrid system / Deyin Guo, Minna-Liisa Rajamäki, and Jari Valkonen

NMR analysis of viral protein structures / Andrew J. Dingley, Inken Lorenzen, and Joachim Grötzinger

Localization of viral proteins in plant cells : protein tagging / Sophie Haupt, Angelika Ziegler, and Lesley Torrance

Construction of infectious clones for RNA viruses : TMV / Sean N. Chapman

Construction of infectious cDNA clones for RNA viruses : turnip crinkle virus / Eugene V. Ryabov

Construction of infectious clones for DNA viruses : mastreviruses / Margaret I. Boulton

Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example / Qi Huang and John S. Hartung. Insertion of introns : a strategy to facilitate assembly of infectious full length clones / I. Elisabeth Johansen and Ole Søgaard Lund

Analysis of cell-to-cell and long-distance movement of apple latent spherical virus in infected plants using green, cyan, and yellow fluorescent proteins / Tsubasa Takahashi and Nobuyuki Yoshikawa

Agroinoculation : a simple procedure for systemic infection of plants with viruses / Zarir E. Vaghchhipawala and Kirankumar S. Mysore

Geminivirus : biolistic inoculation and molecular diagnosis / Anésia A. Santos [and others]

Expression microarrays in plant-virus interaction / Kristina Gruden [and others]

Genome-wide screens for identification of host factors in viral replication / Tadas Panavas [and others]

Phosphorylation of movement proteins by the plasmodesmal-associated protein kinase / Jung-Youn Lee

Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity / Xiaohong Zhu and S.P. Dinesh-Kumar

Yeast two-hybrid assay to identify host-virus interactions / Stuart A. MacFarlane and Joachim F. Uhrig

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Gary Foster,Elisabeth Johansen,Yiguo Hong,Peter Nagy,Plant