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Plasmodesmata Methods and Protocols 1st Edition by Manfred Heinlein 9781493915224 1493915223

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Plasmodesmata Methods and Protocols 1st Edition Manfred Heinlein (Eds.) Digital Instant Download

Author(s): Manfred Heinlein (eds.)
ISBN(s): 9781493915224, 1493915223
Edition: 1
File Details: PDF, 9.46 MB
Year: 2015
Language: english
SKU: EB-4913750 Category: Tag:

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Product details:

ISBN 10: 1493915223

ISBN 13: 9781493915224

Author: Manfred Heinlein

Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.

Table of contents:

Chapter 1. Plasmodesmata: channels for intercellular signaling during plant growth and development

Chapter 2. Plasmodesmata: channels for viruses on the move

Chapter 3. Imaging plasmodesmata with high-resolution scanning electron microscopy

Chapter 4. Preparative methods for imaging plasmodesmata at super-resolution

Chapter 5. Isolation of plasmodesmata from Arabidopsis suspension culture cells

Chapter 6. Immunofluorescence detection of callose deposition around plasmodesmata sites

Chapter 7. Imaging callose at plasmodesmata using aniline blue: quantitative confocal microscopy

Chapter 8. Localization of fluorescently tagged protein to plasmodesmata by correlative light and electron microscopy

Chapter 9. Quantification of plant cell coupling with live-cell microscopy

Chapter 10. Drop-ANd-See: a simple, real-time, and noninvasive technique for assaying plasmodesmal permeability

Chapter 11. Mapping symplasmic fields at the shoot apical meristem using iontophoresis and membrane potential measurements

Chapter 12. Analysis of the conductivity of plasmodesmata by microinjection

Chapter 13. Investigating plasmodesmata genetics with virus-induced gene silencing and an agrobacterium-mediated GFP movement assay

Chapter 14. Probing plasmodesmata function with biochemical inhibitors

Chapter 15. GAL4 transactivation-based assay for the detection of selective intercellular protein movement

Chapter 16. Techniques for assessing the effects of pharmacological inhibitors on intercellular protein movement

Chapter 17. Probing protein targeting to plasmodesmata using fluorescence recovery after photo-bleaching

Chapter 18. Tracking of intercellular small RNA movement

Chapter 19. Analysis of the role of myosins in targeting proteins to plasmodesmata

Chapter 20. Pumilio-based RNA in vivo imaging

Chapter 21. In vivo RNA labeling using MS2

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Tags: Manfred Heinlein, Plasmodesmata, Methods, Protocols