Practical Methods for Biocatalysis and Biotransformations 1st Edition by John Whittall ISBN 0470748591 9780470748596

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Product details:

ISBN 10: 0470748591

ISBN 13: 9780470748596

Author:  John Whittall 

Practical Methods for Biocatalysis and Biotransformations 1st Edition: Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.

Practical Methods for Biocatalysis and Biotransformations is a “how-to” guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include:

• Introduction to the application of biocatalysts in the pharma industry
• introduction to molecular biological techniques for biocatalyst development
• Lipase and protease methods including enzyme supports and dynamic kinetic resolutions
• Nitrile hydrolases and hydratases
• Alcohol dehydrogenases and co-factor recycling
• Epoxide hydrolases
• Amino acid synthesis
• Whole cell processes
• Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases

Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.

 

Practical Methods for Biocatalysis and Biotransformations 1st Edition Table of contents:

 

1. Biotransformations in Small-molecule Pharmaceutical Development

2. Biocatalyst Identification and Scale-up: Molecular Biology for Chemists

3. Kinetic Resolutions Using Biotransformations

   • 3.1 Stereo- and Enantio-selective Hydrolysis of rac-2-Octylsulfate Using Whole Resting Cells of Pseudomonas spp.
   • 3.2 Protease-catalyzed Resolutions Using the 3-(3-Pyridine)propionyl Anchor Group: p-Toluenesulfonamide
   • 3.3 Desymmetrization of Prochiral Ketones Using Enzymes
   • 3.4 Enzymatic Resolution of 1-Methyl-tetrahydroisoquinoline Using Candida rugosa Lipase

4. Dynamic Kinetic Resolution for the Synthesis of Esters, Amides, and Acids Using Lipases

   • 4.1 Dynamic Kinetic Resolution of 1-Phenylethanol by Immobilized Lipase Coupled with In Situ Racemization over Zeolite Beta
   • 4.2 Synthesis of (R)-Butyrate Esters of Secondary Alcohols by Dynamic Kinetic Resolution
   • 4.3 Dynamic Kinetic Resolution of 6,7-Dimethoxy-1-methyl-1,2,3,4-tetrahydroisoquinoline
   • 4.4 Dynamic Kinetic Resolution of Primary Amines with a Recyclable Palladium Nanocatalyst
   • 4.5 Dynamic Kinetic Resolution of Amines Involving Biocatalysis and In Situ Free-radical-mediated Racemization
   • 4.6 Chemoenzymatic Dynamic Kinetic Resolution of (S)-Ibuprofen

5. Enzymatic Selectivity in Synthetic Methods

   • 5.1 Alcalase-catalyzed Syntheses of Hydrophilic Di- and Tri-peptides in Organic Solvents
   • 5.2 Selective Alkoxycarbonylation of 1,25-Dihydroxyvitamin D3 Diol Precursor with Candida antarctica Lipase B
   • 5.3 The Use of Lipase Enzymes for the Synthesis of Polymers and Polymer Intermediates

6. Aldolase Enzymes for Complex Synthesis

   • 6.1 One-step Synthesis of L-Fructose Using Rhamnulose-1-phosphate Aldolase
   • 6.2 Straightforward Fructose-1,6-bisphosphate Aldolase-mediated Synthesis of Aminocyclitols

7. Enzymatic Synthesis of Glycosides and Glucuronides

   • 7.1 Glycosynthase-assisted Oligosaccharide Synthesis
   • 7.2 Glycosyl Azides: Novel Substrates for Enzymatic Transglycosylations

8. Synthesis of Cyanohydrins Using Hydroxynitrile Lyases

   • 8.1 Synthesis of (S)-2-Hydroxy-2-methylbutyric Acid by a Chemoenzymatic Methodology

9. Synthesis of Chiral sec-Alcohols by Ketone Reduction

   • 9.1 Asymmetric Synthesis of (S)-Bis(trifluoromethyl)phenylethanol by Biocatalytic Reduction of Bis(trifluoromethyl)acetophenone

10. Reduction of Functional Groups

11. Enzymatic Oxidation Chemistry

12. Whole-cell Oxidations and Dehalogenations

 

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