Principles and Applications of Fluorescence Spectroscopy 1st Edition by Jihad Rene Albani ISBN 978-1405138918

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ISBN 13: 978-1405138918

Author: Jihad Rene Albani

Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling.

Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.

• Straightforward overview of absorption and fluorescence shows the student how the phenomenon arises and how it can be used in the course of their research.
• Highly practical approach shows non-specialists how to use the technique to investigate chemical and biochemical problems and generate sophisticated results.
• Contains many easy to perform laboratory experiments to reinforce the clear explanations.

Table of contents: 

CHAPTER 1 Absorption Spectroscopy Theory 
CHAPTER 2 Determination of the Calcofluor White Molar Extinction Coefficient Value in the Absence and Presence of α1-Acid Glycoprotein 
CHAPTER 3 Determination of Kinetic Arameters of Lactate Dehydrogenase 
CHAPTER 4 Hydrolysis of p-Nitrophenyl-β-D-Galactoside with β-Galactosidase from E. coli (Pages: 34-58)
CHAPTER 5 Starch Hydrolysis by Amylase
CHAPTER 6 Determination of the pK of a Dye 
CHAPTER 7 Fluorescence Spectroscopy Principles 
CHAPTER 8 Effect of the Structure and the Environment of a Fluorophore on its Absorption and Fluorescence Spectra 
CHAPTER 9 Fluorophore Characterization and Importance in Biology
CHAPTER 10 Fluorescence Quenching 
CHAPTER 11 Fluorescence Polarization 
CHAPTER 12 Interaction Between Ethidium Bromide and DNA 
CHAPTER 13 Lens Culinaris Agglutinin: Dynamics and Binding Studies
CHAPTER 14 Förster Energy Transfer 
CHAPTER 15 Binding of TNS on Bovine Serum Albumin at pH 3 and pH 7 
CHAPTER 16 Comet Test for Environmental Genotoxicity Evaluation: A Fluorescence Microscopy Application 
CHAPTER 17 Questions and Exercises

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