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Protein Chromatography Methods and Protocols 3rd Edition by Sinéad Loughran, John Joseph Milne 9781071633618 1071633619

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Protein Chromatography Methods and Protocols 3rd Edition Sinéad Loughran Digital Instant Download

Author(s): Sinéad Loughran, John Joseph Milne
ISBN(s): 9781071633618, 1071633619
Edition: 3
File Details: PDF, 11.71 MB
Year: 2023
Language: english
SKU: EB-52028328 Category: Tags: ,

Protein Chromatography Methods and Protocols 3rd Edition by Sinéad Loughran, John Joseph Milne – Ebook PDF Instant Download/Delivery: 9781071633618, 1071633619

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Product details:

ISBN 10: 1071633619

ISBN 13: 9781071633618

Author: Sinéad Loughran, John Joseph Milne

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible prools.

Authoritative and cutting-edge, Protein Chromatography: Methods and Prools, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.

Table of contents:

  1. A Synopsis of Proteins and Their Purification

  2. Mixed-mode chromatography and its role in monoclonal antibody purification

  3. Application of Continuous Counter-current Chromatography in Protein Purification

  4. Chromatographic Purification of Viral Vectors for Gene Therapy Applications

  5. Scale-up of Protein Purification – Downstream Processing Issues

  6. Approaches to avoid Proteolysis During Protein Expression and Purification

  7. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification

  8. Magnetic nanoparticles for protein separation and purification

  9. Ion-Exchange Chromatography: Basic Principles and Application

  10. Hydroxyapatite Chromatography

  11. Poly-Histidine-Tagged Protein Purification using IMAC

  12. Lectin Affinity Chromatography

  13. Single-step non-chromatographic purification of recombinant mammalian proteins using a split intein ELP tag system

  14. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems

  15. Immunoprecipitation: Variations, Considerations, and Applications

  16. Protein Quantitation and Analysis of Purity

  17. Protein Extraction and Purification by Differential Solubilisation

  18. Protein Stability: Enhancement and Measurement

  19. Storage and Lyophilisation of Pure Proteins

  20. Strategies for the Purification of Membrane Proteins

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Tags: Sinéad Loughran, John Joseph Milne, Protein, Chromatography