Protein Purification Protocols Methods in Molecular Biology Vol 244 2nd Edition by Paul Cutler 159259655X 9781592596553

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ISBN 10: 159259655X 

ISBN 13: 9781592596553

Author: Paul Cutler

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Table of contents:

General strategies / S. Doonan and P. Cutler

Preparation of extracts from animal tissues / J.M. Skehel

Protein extraction from plant tissues / R.J. Fido [and others]

Extraction of recombinant protein from bacteria / A.F. McGettrick and D.M. Worrall

Protein extraction from fungi / P.D. Bridge, T. Kokubun and M.S. Simmonds

Subecllular fractionation of animal tissues / N.M. Ryan

Subcellular fractionation of plant tissues: Isolation of plastids and mitochondria / A.K. Tobin and C.G. Bowsher

The extraction of enzymes from plant tissues rich in phenolic compounds / W.S. Pierpoint

Avoidance of proteolysis in extracts / R.J. Beynon and S. Oliver

Concentration of extracts / S. Doonan

Making and changing buffers / S. Doonan

Purification and concentration by ultrafiltration / P. Schratter

Bulk purification by fractional precipitation / S. Doonan

Ion-exchange chromatography / C. Selkirk

Hydrophobic interaction chromatography / P.A. O’Farrell

Affinity chromatography / P. Cutler

Dye-ligand affinity chromatography / A.F. McGettrick and D.M. Worrall

Lectin affinity chromatography / I. West and O. Goldring

Immunoaffinity chromatography / P. Cutler

Immobilized metal-ion affinity chromatography / T.T. Yip and T.W. Hutchens

Chromatography on hydroxyapatite / S. Doonan

Thiophilic affinity chromatography and related methods / P. Matejtschuk

Affinity precipitation methods / J.A. Irwin and K.F. Tipton

Isoelectric focusing / R. Westermeier

Chromatofocusing / T.J. Mantle and P. Noone

Size-exclusion chromatography / P. Cutler

Fast protein liquid chromatography / D. Sheehan and S. O’Sullivan

Reversed-phase chromatography of proteins / W.A. Neville

Extraction of membrane proteins / K. Ohlendieck

Removal of detergent from protein fractions / K. Ohlendieck

Purification of membrane proteins / K. Ohlendieck

Lyophilization of proteins / C. O’Fagain

Storage of pure proteins / C. O’Fagain

Electroelution of proteins from polyacrylamide gels / M.J. Dunn

Electroblotting of proteins from polyacrylamide gels / M.J. Dunn

Two-dimensional polyacrylamide gel electrophoresis for proteome analyses / N.A. Jones

Microscale solution isoelectrofocusing: A sample prefractionation method for comprehensive proteome analysis / X. Zuo and D.W. Speicher

Practical column chromatography / S. Doonan

Detection methods / J. Mozdzanowski and S. Burman

Peptide proteomics / D.E. McNulty and J.R. Slemmon

Multidimensional liquid chromatography of proteins / R. Watson and T. Nadler

Mass spectrometry / D.J. Bell

Purification of therapeutic proteins / J. Bonnerjea

Purification process scale-up / K. Prince and M. Smith

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Tags: Paul Cutler, Protein, Purification, Protocols, Methods