Quantitation of amino acids and amines by chromatographymethods and protocols 1st Edition by Ibolya Molnár Perl 0444520503 9780444520500

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ISBN 10: 0444520503

ISBN 13: 9780444520500

Author: Ibolya Molnár-Perl

Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC).
The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines.

* Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes
* Compares advantages/disadvantages of various chromatographic techniques
* Readers can undertake practical tasks using detailed protocols given in the book

Table of contents:

Part 1. Amino Acids
1.1. Gas Chromatography
Chapter 1.1.1: Quantitation of Amino Acids as Chloroformates – A Return to Gas Chromatography
Chapter 1.1.2: Quantitation of Amino Acids by Gas-Liquid Chromatography
Chapter 1.1.3: Chiral Separation of Amino Acids by Gas Chromatography

1.2. High Performance Liquid Chromatography
Chapter 1.2.1: HPLC of Amino Acids without Derivatization
Chapter 1.2.2: HPLC of Amino Acids as Phenylthiocarbamoyl Derivatives
Chapter 1.2.3: HPLC of Amino Acids as o-Phthalaldehyde Derivatives
Chapter 1.2.4: HPLC of Amino Acids as Chloroformate Derivatives
Chapter 1.2.5: HPLC of Amino Acids as Dansyl and Dabsyl Derivatives
Chapter 1.3.6: Quantitation of Amino Acids as 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatives

1.3. Capillary Electrophoresis/Capillary Electrochromatography
Chapter 1.3.1: Determination of Underivatized Amino Acids by Capillary Electrophoresis and Capillary Electrochromatography
Chapter 1.3.2: Quantitation of Amino Acids as o-Phthalaldehyde Derivatives by CE
Chapter 1.3.3: Capillary Electrophoresis and Capillary Electrochromatography of Amino Acids as Dansyl Derivatives

Part 2. Amines
2.1. Gas Chromatography
Chapter 2.1.1: Gas Chromatographic Determination of Volatile Aliphatic and Selected Aromatic Amines, without Derivatization: Solid Phase Microextraction
Chapter 2.1.2: Gas Chromatography of Amines as Various Derivatives

2.2. High Performance Liquid Chromatography
Chapter 2.2.1: HPLC of Amines as o-Phthalaldehyde Derivatives
Chapter 2.2.2: Quantitation by HPLC of Amines as Dansyl Derivatives
Chapter 2.2.3: HPLC of Amines as 9-Fluorenylmethyl Chloroformate Derivatives
Chapter 2.2.4: HPLC of Biogenic Amines as 6-Aminoquinolyl-N-hydroxysuccinimidyl Derivatives

2.3. Capillary Electrophoresis/Capillary Chromatography
Chapter 2.3.1: Determination of Underivatized Amines by Capillary Electrophoresis and Capillary Electrochromatography
Chapter 2.3.2: Quantitation of Amines by Oncolumn Derivatization with o-Phthalaldehyde by Capillary Electrochromatography

3. Quantitation of Amino Acids and Amines, Simultaneously
Chapter 3: Quantitation of Amino Acids and Amines, Simultaneously

4. Quantitation of Polyamines by Chromatography
Chapter 4: Chromatography of Polyamines

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Tags: Ibolya Molnár Perl, Quantitation, chromatographymethods, amino