Regulators of G Protein Signaling Part A 1st Edition by David Siderovski 0121827941 9780121827946

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ISBN 10: 0121827941

ISBN 13: 9780121827946

Author: David P. Siderovski

This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.

Table of contents:

Section I: General Methods of RGS Protein Analysis
Subsection A. Expression and Post-translational M
Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein S
Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins
Chapter 3. Role of Palmitoylation in RGS Protein Function
Subsection B: Assays of RGS Box Activity and Allosteric Control
Chapter 4. Fluorescence-Based Assays for RGS Box Function
Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Si
Chapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signali
Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin
Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits
Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling
Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels
Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha Subunits
Chapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Prot
Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expre
Subsection D. Mouse Models of RGS Protein Action
Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS
Chapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins
Subsection E. Methods of RGS Protein Inhibition
Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins
Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activi
Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins

Section II: G-Protein Regulators of Model Organisms
Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans
Chapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G
Chapter 20. AtRGS1 Function in Arabidopsis thaliana
Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco
Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Dr
Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast
Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response

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Tags: David Siderovski, Regulators, Protein, Signaling