Section I: Investigating Solution Chemistry, Structure, and Nucleation
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Chapter One. Development of Accurate Force Fields for the Simulation of Biomineralization
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Chapter Two. The Integration of Ion Potentiometric Measurements with Chemical, Structural, and Morphological Analysis to Follow Mineralization Reactions in Solution
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Chapter Three. Investigating the Early Stages of Mineral Precipitation by Potentiometric Titration and Analytical Ultracentrifugation
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Chapter Four. Replica Exchange Methods in Biomineral Simulations
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Chapter Five. SAXS in Inorganic and Bioinspired Research
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Chapter Six. In Situ Solution Study of Calcium Phosphate Crystallization Kinetics
Section II: Probing Structure and Dynamics at Surfaces and Interfaces
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Chapter Seven. Design, Fabrication, and Applications of In Situ Fluid Cell TEM
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Chapter Eight. X-ray Absorption Spectroscopy for the Structural Investigation of Self-Assembled-Monolayer-Directed Mineralization
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Chapter Nine. Cryo-TEM Analysis of Collagen Fibrillar Structure
Section III: Biomimetic Crystallization Techniques in vitro
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Chapter Ten. Preparation of Organothiol Self-Assembled Monolayers for Use in Templated Crystallization
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Chapter Eleven. Experimental Techniques for the Growth and Characterization of Silica Biomorphs and Silica Gardens
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Chapter Twelve. Precipitation in Liposomes as a Model for Intracellular Biomineralization
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Chapter Thirteen. Polymer-Mediated Growth of Crystals and Mesocrystals
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Chapter Fourteen. Phage Display for the Discovery of Hydroxyapatite-Associated Peptides
Section IV: Protein Structure, Interactions and Function
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Chapter Fifteen. Quantitatively and Kinetically Identifying Binding Motifs of Amelogenin Proteins to Mineral Crystals Through Biochemical and Spectroscopic Assays
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Chapter Sixteen. Using the RosettaSurface Algorithm to Predict Protein Structure at Mineral Surfaces
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Chapter Seventeen. Investigating Protein Function in Biomineralized Tissues Using Molecular Biology Techniques
Section V: Mapping Biomineral Morphology and Ultrastructure
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Chapter Eighteen. Imaging the Nanostructure of Bone and Dentin Through Small- and Wide-Angle X-Ray Scattering
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Chapter Nineteen. Synchrotron X-Ray Nanomechanical Imaging of Mineralized Fiber Composites
Section VI: Mapping Mineral Chemistry
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Chapter Twenty. Application of Total X-Ray Scattering Methods and Pair Distribution Function Analysis for Study of Structure of Biominerals
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Chapter Twenty-One. X-Ray Microdiffraction of Biominerals
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Chapter Twenty-Two. FTIR and Raman Studies of Structure and Bonding in Mineral and Organic–Mineral Composites
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Chapter Twenty-Three. A Mixed Flow Reactor Method to Synthesize Amorphous Calcium Carbonate Under Controlled Chemical Conditions