Reverse Chemical Genetics Methods and Protocols 1st Edition by Osamu Ohara 1607612321 9781607612322

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ISBN 10: 1607612321 
ISBN 13: 9781607612322 
Author: Osamu Ohara 

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.

Reverse Chemical Genetics Methods and Protocols 1st Table of contents:

The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics
Pages: 1

ORFeome Cloning
Pages: 3–9

Systematic Cloning of an ORFeome Using the Gateway System
Pages: 11–24

High-Throughput Construction of ORF Clones for Production of Recombinant Proteins
Pages: 25–39

Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study
Pages: 41–54

High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data
Pages: 55–65

Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology
Pages: 67–79

Modification of cDNA Resources Utilized for Reverse Chemical Genetics
Pages: 82

High-Throughput Production of Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources
Pages: 83–96

An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources
Pages: 97–108

Recombinant Protein Production by a Kaiko–Baculovirus System
Pages: 109–120

Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins
Pages: 121–131

The Refinement of Established Methods by Recent Innovations
Pages: 134

Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
Pages: 135–154

Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis
Pages: 155–166

Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos
Pages: 167–179

Identification of the Specific Binding Proteins of Bioactive Small Compounds Using Affinity Resins
Pages: 181–195

Potential Applicable Techniques for Reverse Chemical Genetics
Pages: 198

Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases

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Osamu Ohara,Reverse Chemical,Genetics Methods