RNA and DNA Editing Methods and Protocols 1st Edition by Ruslan Aphasizhev 1617790176 9781617790171

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Product details:

ISBN 10: 1617790176

ISBN 13: 9781617790171

Author: Ruslan Aphasizhev

From the reviews:It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor … has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques … . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab. (Emanuele Buratti, Molecular Biotechnology, September, 2011), From the reviews: “This book on RNA and DNA Editing, Methods and Protocols provides methods to study editing substrates, mechanisms of specificity and functions of RNA and DNA editing enzymes and complexes … . The apparent audience is molecular biologists interested in RNA and DNA editing.” (Omer Iqbal, Doody’s Book Reviews, March, 2012) “It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor … has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques … . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab.” (Emanuele Buratti, Molecular Biotechnology, September, 2011), From the reviews: This book on RNA and DNA Editing, Methods and Protocols provides methods to study editing substrates, mechanisms of specificity and functions of RNA and DNA editing enzymes and complexes … . The apparent audience is molecular biologists interested in RNA and DNA editing. (Omer Iqbal, Doody’s Book Reviews, March, 2012) It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor … has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques … . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab. (Emanuele Buratti, Molecular Biotechnology, September, 2011)

Table of contents:

Part I: Uracil Insertion/Deletion RNA Editing in Mitochondrion of Trypanosoma brucei

1. Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy

2. iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei Gene

Part II: Adenosine to Inosine RNA Editing
3. Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination
4. Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis
5. Biochemical Identification of A-to-I RNA Editing Sites by the Inosine Chemical Erasing (ICE) Method

Part III: Cytidine to Uracil RNA and DNA Editing
6. Identifying mRNA Editing Deaminase Targets by RNA-Seq
7. Mouse and Other Rodent Models of C to U RNA Editing
8. In Vivo Analysis of RNA Editing in Plastids
9. Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing
10. Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection
11. A High Throughput Assay for DNA Deaminases
12. Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes

Part IV: tRNA Editing and RNA Modifications
13. Analysis of tRNA Editing in Native and Synthetic Substrates
14. Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs
15. Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target
16. A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes

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Tags: Ruslan Aphasizhev, Editing, Methods, Protocols