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RNA Scaffolds Methods and Protocols 2nd Edition by Luc Ponchon 1071614983 9781071614983

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RNA Scaffolds Methods and Protocols 2nd Edition Luc Ponchon Digital Instant Download

Author(s): Luc Ponchon
ISBN(s): 9781071614983, 1071614983
Edition: 2
File Details: PDF, 7.25 MB
Year: 2021
Language: english
SKU: EB-32414178 Category: Tag:

RNA Scaffolds Methods and Protocols 2nd Edition by Luc Ponchon – Ebook PDF Instant Download/Delivery: 1071614983, 9781071614983

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Product details:

ISBN 10: 1071614983 

ISBN 13: 9781071614983

Author: Luc Ponchon

This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field.

Table of contents:

  1. Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA

  2. RNA Footprinting Using Small Chemical Reagents

  3. Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment

  4. Using tRNA Scaffold to Assist RNA Crystallization

  5. RNA Modeling with the Computational Energy Landscape Framework

  6. Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli

  7. In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold

  8. Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds

  9. Identification of RNA-Binding Proteins Associated to RNA Structural Elements

  10. Live Cell Imaging Using Riboswitch–Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors

  11. Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging

  12. Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria

  13. FRET Analysis of RNA–Protein Interactions Using Spinach Aptamers

  14. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

  15. Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells

  16. Folding RNA–Protein Complex into Designed Nanostructures

  17. An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA

  18. Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs

  19. Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics

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Tags: Luc Ponchon, Scaffolds, Methods, Protocols