Stem Cell Renewal and Cell Cell Communication Methods and Protocols 1st Edition by Kursad Turksen ISBN 149392589X 9781493925896

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ISBN 10: 149392589X 
ISBN 13: 9781493925896
Author: Kursad Turksen

This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Stem Cell Renewal and Cell Cell Communication Methods and Protocols 1st Table of contents:

• Laboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal

  • (Covers fundamental techniques for maintaining stem cell cultures in a self-renewing state)

• Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche

  • (Details advanced imaging techniques to visualize the microenvironment of stem cells)

• Multiplex Immunoassays for Quantification of Cytokines, Growth Factors and Other Proteins in Stem Cell Communication

  • (Describes methods for measuring secreted factors involved in cell-cell signaling)

• Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells

  • (Presents a specific culture method for expanding human pluripotent stem cells)

• Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

  • (Focuses on optimizing conditions for maintaining and validating rat embryonic stem cells)

• Generation, Expansion and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)

  • (Provides protocols for deriving and differentiating specific stem cell lineages)

• Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells

  • (Methods for working with mouse-derived neural stem cells)

• Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells

  • (Protocols for generating cardiovascular cells from hPSCs)

• A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon

  • (Specific methods for isolating and culturing mesenchymal stem cells from particular tissues)

• A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function

  • (Describes a system for studying stem cell behavior in a specific organoid model)

• Ca2+ Handling in Mouse Embryic Stem Cell Derived Cardiomyocytes

  • (Focuses on a specific functional aspect of differentiated stem cells)

• Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer’s Disease Therapeutics

  • (Explores therapeutic applications and the role of intercellular communication via extracellular vesicles)

• Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells

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Tags: Kursad Turksen, Stem, Cell