Steroid Receptors Methods and Protocols 1st Edition by Gabriella Castoria, Ferdinando Auricchio 9781493913466 1493913468
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Product details:
ISBN 10: 1493913468
ISBN 13: 9781493913466
Author: Gabriella Castoria, Ferdinando Auricchio
Steroid Receptors: Methods and Prools presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Steroid Receptors: Methods and Prools offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.
Table of contents:
Chapter 1. Progesterone Receptor Interaction with Chromatin
Chapter 2. Mapping the Genomic Binding Sites of the Activated Retinoid X Receptor in Murine Bone Marrow-Derived Macrophages using Chromatin Immunoprecipitation Sequencing
Chapter 3. Analysis of Chromatin-Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation
Chapter 4. Examining Estrogen Regulation of Cancer Stem Cells through Multicolor Lineage Tracing
Chapter 5. Reporter Mice for the Study of Long Term Effects of Drugs and Toxic Compounds
Chapter 6. Analysis of Histone post-Translational Modifications in the Control of Chromatin Plasticity Observed at Estrogen-Responsive Sites in Human Breast Cancer Cells
Chapter 7. In Silico Analysis of Genomic Data for Construction of Nuclear Receptor Network
Chapter 8. Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment
Chapter 9. Paxillin and Steroid Signaling: from Frog to Human
Chapter 10. Analysis of the Androgen Receptor/Filamin A Complex in Stromal Cells
Chapter 11. Multi-Well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membrane Vs. Intracellular Receptor Levels
Chapter 12. Promixity Ligation Assay to Detect and Localize the Interactions of ERa with PI3-K and Src in Breast Cancer Cells and Tumor Samples
Chapter 13. Phosphoinositide 3-Kinase Assay in Breast Cancer Cell Extracts
Chapter 14. Rapid Estrogen Effects on Aromatase Phosphorylation in Breast Cancer Cells
Chapter 15. Mouse Monoclonal Antibodies against Estrogen Receptor
Chapter 16. Analysis of Estrogen Receptor Interacting Proteins Using Pull Down Assay and MALDI-MS Methods
Chapter 17. Analysis of the Conformation of the Androgen Receptor in Spinal Bulbar Muscular Atrophy by Atomic Force Microscopy
Chapter 18. Imaging of Corticosteroid Receptors in Live Cells
Chapter 19. Physiological Techniques in the Study of Rapid Aldosterone Effects
Chapter 20. Detection of the Glucocorticoid Receptors in Brain Protein Extracts by SDS-PAGE
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Tags: Gabriella Castoria, Ferdinando Auricchio, Steroid, Receptors