T Cell Protocols 2nd Edition by Angus Stock, Vincenzo Cerundolo, Gennaro De Libero 1588295877 9781588295873

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ISBN 10: 1588295877 

ISBN 13: 9781588295873

Author: Angus Stock, Vincenzo Cerundolo, Gennaro De Libero

This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters old techniques, which remain fundamental to T cell immunology, are described in their modern versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficacious descriptions of novel methods.

T Cell Protocols 2nd Table of contents:

Analysis of frequency and phenotype of antigen-specific T cells / Angus Stock and Vincenzo Cerundolo
B cell helper assays / Sergio Abrignani, … [et al.]Transkingdom RNA interference (tkRNAi) / Thu A. Nguyen and Johannes H. Fruehauf
Flow cytometry and cell activation / Sonia Gavasso
Investigating T cells by polychromatic flow cytometry / Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza
Generation of human T cell clones / Sabrina Mariotti and Roberto Nisini
Limiting dilution analysis of antigen-specific T cells / Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan
T cell epitope-mapping by cytokine gene expression assay / Maurizio Provengano and Giulio C. Spagnoli
Cytokine multiplex immunoassay / Wilco de Jager, Berent Prakken, and Ger T. Rijkers
Purification of the T cell antigen receptor and analysis by blue-native PAGE / Mahima Swamy and Wolfgang W.A. Schamel
Non-replicating recombinant vaccinia virus expressing CD80 to enhance T-cell stimulation / Paul Zajac.

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Angus Stock,Vincenzo Cerundolo,Gennaro De Libero,Protocols