The Metabolic Pathway Engineering Handbook Two Volume Set 1st Edition by Christina Smolke 9781420005622 1420005626
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Product details:
ISBN 10: 1420005626
ISBN 13: 9781420005622
Author: Christina D. Smolke
Christina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metab
Table of contents:
Volume I
SECTION I: Evolutionary Tools in Metabolic Engineering
1 Evolutionary Engineering of Industrially Important Microbial Phenotypes
2 Improving Protein Functions by Directed Evolution
3 Engineering DNA and RNA Regulatory Regions through Random Mutagenesis and Screening
4 Evolving Pathways and Genomes for the Production of Natural and Novel Compounds
5 Models Predicting Optimized Strategies for Protein Evolution
SECTION II: Gene Expression Tools for Metabolic Pathway Engineering
6 Low-Copy Number Plasmids as Artificial Chromosomes
7 Chromosomal Engineering Strategies
8 Regulating Gene Expression through Engineered RNA Technologies
9 Tools Designed to Regulate Translational Efficiency
10 Metabolic Engineering of the Secretory Processing Pathway in Eukaryotes
11 Engineering Multifunctional Enzyme Systems for Optimized Metabolite Transfer between Sequential C
12 Practical Pathway Engineering—Demonstration in Integrated Tools
SECTION III: Application of Emerging Technologies to Metabolic Engineering
13 Genome-Wide Technologies: DNA Microarrays, Phenotypic Microarrays, and Proteomics
14 Monitoring and Measuring the Metabolome
SECTION IV: Future Prospects in Metaboli Engineering
15 Systems Biology, Genome-Scale Models, and Metabolic Engineering
16 Cell-Free Systems for Metabolic Engineering
17 In Silico Models for Metabolic Systems Engineering
SECTION V: Tools for Experimentally Determining Flux through Pathways
18 GC–MS for Metabolic Flux Analysis
19 Tools for Measuring Intermediate and Product Formation
20 Use of AEX-HPLC-ESI-MS for [sup(13)]C-Labeling Based Metabolic Flux Analysis in Saccharomyces cer
SECTION VI: Future Applicationf of Meabolic Engineering
21 Energy and Cofactor Issues in Fermentation and Oxyfunctionalization Processes
22 Microbial Biosynthesis of Fine Chemicals: An Emerging Technology
23 Applications of Metabolic Engineering for Natural Drug Discovery
24 Metabolic Engineering for Alternative Fuels
Volume II
SECTION I: Cellular Metabolism
1 Solute Transport Processes in the Cell
2 Catabolism and Metabolic Fueling Processes
3 Biosynthesis of Cellular Building Blocks: The Prerequisites of Life
4 Polymerization of Buliding Blocks to Macromolecules Polyhydroxyalkanoates as an Example
5 Rare Metabolic Conversions—Harvesting Diversity through Nature
SECTION II: Balances and Reaction Models
6 Growth Nutrients and Diversity
7 Mass Balances, Rates, and Experiments
8 Data Reconciliation and Error Detection
9 Black Box Models for Growth and Product Formation
10 Metabolic Models for Growth and Product Formation
11 A Thermodynamic Description of Microbial Growth and Product Formation
SECTION III: Bacterial Transcriptional Regulation of Metabolism
12 Transcribing Metabolism Genes: Lessons from a Feral Promoter
13 Regulation of Secondary Metabolism in Bacteria
14 A Synthetic Approach to Transcriptional Regulatory Engineering
Chpater IV. Modeling Tools for Metabolic Engineering
15 Metabolic Flux Analysis
16 Metabolic Control Analysis
17 Structure and Flux Analysis of Metabolic Networks
18 Constraint-Based Genome-Scale Models of Cellular Metabolism
19 Multiscale Modeling of Metabolic Regulation
20 Validation of Metabolic Models
SECTION V: Developing Appropriate Hosts for Metabolic Engineering
21 Escherichia coli as a Well-Developed Host for Meabolic Engineering
22 Metabolic Engineering in Yeast
23 Metabolic Engineering of Bacillus subtilis
24 Metabolic Engineering of Streptomyces
25 Metabolic Engineering of Filamentous Fungi
26 Metabolic Engineering of Mammalian Cells
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Tags: Christina Smolke, Metabolic, Pathway, Engineering