The Nuclear Receptor Superfamily Methods and Protocols 1st Edition by Iain J Mcewan ISBN 1603275746 9781603275743

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ISBN 10: 1603275746 
ISBN 13: 9781603275743
Author: Iain J Mcewan

Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.

The Nuclear Receptor Superfamily Methods and Protocols 1st Table of contents:

Part I: General Aspects and Introduction

• 1. The Nuclear Receptor Superfamily at Thirty (This is a introductory chapter likely providing an overview and historical context of the field.)

  • Authored by Iain J. McEwan

  • This chapter covers the basic principles of nuclear receptors and their physiological roles.

Part II: Nuclear Receptor Agonists and Antagonists

• 2. Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization

• 3. Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands

• 4. Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor

Part III: Nuclear Receptor Signalling

• 5. Use of BRET to Study Protein–Protein Interactions In Vitro and In Vivo

• 6. Studying Nuclear Receptor Complexes in the Cellular Environment

• 7. Posttranslational Modifications of Steroid Receptors: Phosphorylation

• 8. Mapping Protein–DNA Interactions Using ChIP-exo and Illumina-Based Sequencing

• 9. Methods to Identify Chromatin-Bound Protein Complexes: From Genome-Wide to Locus-Specific Approaches

• 10. Measuring the Expression of microRNAs Regulated by Androgens

• 11. Methods for Identifying and Quantifying mRNA Expression of Androgen Receptor Splicing Variants in Prostate Cancer

Part IV: Model Systems and in vivo imaging

• 12. Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

• 13. In Vivo Imaging of Nuclear Receptor Transcriptional Activity

• 14. Development and Characterization of Cell-Specific Androgen Receptor (and likely other chapters in this part)

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Tags: Iain J Mcewan, Nuclear, Receptor