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Therapeutic Oligonucleotides Methods and Protocols 1st Edition by John Goodchild 9781617791888 1617791881

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Therapeutic Oligonucleotides Methods and Protocols 1st Edition John Goodchild (Auth.) Digital Instant Download

Author(s): John Goodchild (auth.), John Goodchild (eds.)
ISBN(s): 9781617791888, 1617791881
Edition: 1
File Details: PDF, 5.68 MB
Year: 2011
Language: english
SKU: EB-2450056 Category: Tags: ,

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Product details:

ISBN 10: 1617791881

ISBN 13: 9781617791888

Author: John Goodchild

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.

Table of contents:

Chapter 1: Therapeutic Oligonucleotides
Chapter 2: Dinucleotides Containing 3′-S-Phosphorothiolate Linkages
Chapter 3: 2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
Chapter 4: A Non-covalent Peptide-based Strategy for Ex-vivo and In vivo Oligonucleotide Delivery
Chapter 5: Cell Penetrating Peptides-based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides
Chapter 6: A Nanoparticle for Tumor Targeted Delivery of Oligomers
Chapter 7: Light-directed Delivery of Nucleic Acids
Chapter 8: Antibody Targeted siRNA Delivery
Chapter 9: Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy
Chapter 10: Five-step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
Chapter 11: Diverse Small Non-coding RNAs in RNA Interference Pathways
Chapter 12: Quantification of siRNAs In vitro and In vivo
Chapter 13: Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR
Chapter 14: siRNA Knockdown of Gene Expression in Endothelial Cells
Chapter 15: Using RNA Interference in Schistosoma mansoni
Chapter 16: Performing the Labeled microRNA Pull-down (LAMP) Assay System: An Experimental Approach for High-throughput Identification of microRNA-target mRNAs
Chapter 17: Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
Chapter 18: Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-like Receptor 9
Chapter 19: Surface Plasmon Resonance Investigation of RNA Aptamer-RNA Ligand Interactions
Chapter 20: Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
Chapter 21: Inhibition of Human Papillomavirus Expression Using DNAzymes

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Tags: John Goodchild, Therapeutic, Oligonucleotides, Methods