Transgenic Animal Technology A Laboratory Handbook 3rd Edition by Carl Pinkert 0124104908 9780124104907

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ISBN 10: 0124104908

ISBN 13: 9780124104907

Author: Carl A. Pinkert

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Transgenic Animal Technology A Laboratory Handbook 3rd Table of contents:

1. Introduction to Transgenic Animal Technology
I Introduction
II Historical Background
III Applications and Overview of Text
References

Section Two: Transgenic Animal Production Focusing on the Mouse Model
2. DNA Microinjection, Embryo Handling, and Germplasm Preservation
I Introduction
II General Methods
III Summary
Acknowledgments
References
Appendix

3. Factors Affecting Transgenic Animal Production
I Introduction
II Getting Started
III Troubleshooting
IV Transgenic Phenomenology
V Summary
Acknowledgments
References

4. Gene Targeting in Embryonic Stem Cells, I: History and Methodology
I Stem Cells of the Mouse Embryo
II Gene Targeting in ESCs
III Methods
References

5. Gene Targeting in Embryonic Stem Cells, II: Conditional Technologies
I Introduction
II Conditional Modeling: A Brief History
III Recombinase Systems in ES Cell Modification
IV Methods
Abbreviations
References

6. Retrovirus-Mediated Gene Transfer
I Retroviruses
II Strategies of Retrovirus Vector Systems
III Problems in the Use of Retrovirus Vectors in Gene Transfer
IV Methods
Acknowledgments
References

7. Nuclear Transfer Technologies
I Introduction and Discussion
II Materials and Equipment
III Preparation of Microtools
IV Nuclear Transfer of Preimplantation Embryos
V Nuclear Transfer of Embryonic Stem Cells and Somatic Cells
Acknowledgments
References

8. Gene Editing
I Introduction
II Sequence Modifications in Genome Editing
III Approaches to Genome Editing
IV Online Resources
V Commercial Resources
VI Summary
References

Section Three: Production of Transgenic Laboratory and Domestic Animal Species
9. Production of Transgenic Rats
I Introduction
II Importance of Rats
III Contributions from Transgenic Rats
IV Production
V Summary
Acknowledgments
References

10. Production of Transgenic Rabbits
I Introduction—Rabbits in Biomedicine
II Overview of Methods for Producing Genetically Modified Rabbits
III Materials and Methods
IV Conclusion
References

11. Production of Transgenic Fish
I Introduction
II Discussion
III Methods of Gene Transfer
IV Establishing and Maintaining Transgenic Lineages
V Gene Knockout
VI Performance of Transgenic Aquaculture-Generated Species of Fish
VII Care and Containment of Transgenic Fish
VIII Future of Transgenic Fish
References

12. Production of Transgenic Poultry
I Introduction and Discussion
II Methods
III Summary
Acknowledgments
References

13. Production of Transgenic Nonhuman Primates
I Introduction
II Methods
III Summary
Acknowledgments
References

14. Nuclear Transfer Technology in Cattle, Sheep, and Swine
I Introduction
II Protocol for Bovine NT
III Protocol for Porcine NT
IV Protocol for Ovine NT
V Summary
References

15. Alternative Methods for Transgenesis in Domestic Animal Species
I Introduction and Discussion
II Methods
III Alternative Methods for the Production of Transgenic Swine
IV Summary
References

Section Four: Molecular Biology, Analyses and Enabling Technologies
16. Analysis of Phenotype
I Introduction
II Methods for Robust, Relevant, and Reproducible Phenotyping
III Conclusions
References
Appendix
References in Order of Citation in Table A.16.1

17. Design of Vectors for Optimizing Transgene Expression
I Introduction
II Basic Vectors
III Vectors Using Long Genomic DNA Fragments
IV Gene Targeting
V Specific Inhibition Gene Expression
VI Relations between Vectors and Gene Transfer Techniques
VII Conclusion
References

18. Analysis of Transgene Integration
I Introduction
II Chromosomal Position Effects
III Promoting the Integration at Selected Genomic Regions
IV Discussion
V Conclusion and Perspectives
Acknowledgments
References

19. PCR Optimization for Detection of Transgene Integration
I Introduction
II Discussion
III Summary
References

20. Analysis of Transgene Expression
I Introduction
II Analyses of Steady-State Levels of Transgenic RNA Transcripts
III Analyses of Steady-State Levels of Transgenic Protein Products
IV Conclusion
Acknowledgment
References

21. Compound Transgenics: Recombinase-Mediated Gene Stacking
I Introduction
II Gene-Stacking Strategy
III Materials and Methods
IV Summary
V Notes
References

22. Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals
I Introduction
II Assisted Reproductive Technologies
III Embryo Culture Systems
IV Embryo Coculture Systems
V The Use of Embryo Culture Systems
VI Additional Assisted Reproductive Technologies
VII Summary and Future Direction
References

23. Modifying Mitochondrial Genetics
I Introduction
II Methods
Acknowledgments
References

24. Databases, Internet Resources, and Genetic Nomenclature
I Introduction
II Transgenic, Targeted, and Gene Trap Mutation Databases and Online Resources
III Standardized Nomenclature for Transgenic, Targeted, and Gene Trap Mutation Animals

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