Transgenic Mouse Methods and Protocols 1st Edition by Melissa Larson 1493998366 9781493998364

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ISBN 10: 1493998366

ISBN 13: 9781493998364

Author: Melissa A. Larson

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blasyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Transgenic Mouse: Methods and Prools is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.

Transgenic Mouse Methods and Protocols 1st Table of contents:

The History of Transgenesis

Pronuclear Microinjection of One-Cell Embryos

Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection

In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos

Targeted Mutations in the Mouse via Embryonic Stem Cells

Blastocyst Microinjection with Embryonic Stem Cells

Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos

Embryo Transfer Surgery

Non-Surgical Embryo Transfer Protocol for Use with the NSET™ Device

Nuclear Transfer and Cloning

Sperm-Mediated Genetic Modifications

Genotyping Genetically Modified (GM) Mice

Nomenclature: Naming Your Gene-Modified Mouse

Breeding Strategies for Genetically Modified Mice

Strategies for Behaviorally Phenotyping the Transgenic Mouse

Cryobanking and Recovery of Genetically Modified Mice

Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques

Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus

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